Vol. 3 & Issue 2 ; Section B : Biological Sciences

Date : Feb. to Apr.2013

 

A new species of genus Cyana Walker (Lepidoptera: Arctiidae: Lithosiinae) from India

Jagbir Singh Kirti, Rahul Joshi & Navneet Singh

  • Abstract

    A new species, Cyana pseudoeffracta sp. nov. is described from Meghalaya (India). In addition to, external genitalic structures of three other species viz., C. quadrinotata (Walker), C. bellissima (Moore) and C. harterti (Elwes) are described. An updated checklist of Indian species of Cyana Walker is also given.

Status of Lactic Acid Bacteria in Lassi

Y.D. Deshmukh ,Shraddha Bajaj, A.L. Shirfule and Supriya Beedkar

  • Abstract

    Storage studies were carried out for lactic count of Lassi stored at 37± 20C and 7± 10C. At storage temperature 37± 20C, the Lactic acid bacteria (LAB) count found in observed to cause about four log cycle reduction in LAB, while the survivor of Lactic bacteria where still lower in Lassi subjected to thermization at higher temperature (L70), The product stored at 7± 1c, the control sample found about 1.7 log cycle reduction compared to initial count on 15th day of storage and then tended to increase marginally up to 30th, whereas LAB count found slight increase after 15th days onwards till 35th day for the L70 product.

Effects of stocking density on hematological parameters, growth and survival rate of Caspian Roach (Rutilus rutilus caspicus) larvae

Sohrab Ahmadivand, Soheil Eagderi, Mohammad Reza Imanpour

  • Abstract

    Hematological parameters and growth performance are considered as indicators to determine the response of fish to different environmental conditions. Stocking density in aquaculture is one of the important stressors that can effect on these indicators. This study was conducted to determine the impact of different stocking density on the hematological parameters, growth performance and survival rate of Caspian Roach larvae as well as to determine its appropriate stocking density. In total, 540Caspian Roach larvae with average weight of 0.327±0.02 g were introduced to 30 liter tanks in stocking density of 1, 2 and 3 larvae per liter with three replicates. The differences in growth performance including weight gain (WG), feed conversion ratio (FCR) and specific growth rate between the three treatments (P<0.05), indicating the decrease of growth performance with the increasing stocking density. Survival rate of treatments was 100%. Despite of slight increase of some hematological parameters including hematocrit, hemoglobin, red blood cells, white blood cells, MCH, MCHC, MCV, eosinophils, heterophile, monocytes and lymphocytes, no significant difference optimum stocking density of one larva per liter is suggested.

Comparison of microscopic, macromorphological and aflatoxin producing capabilities of Aspergillus species associated with rhizosphere of groundnut (A. hypogaea L.)

Manjusha Chakranarayan and Anita Pati

  • Abstract

    The role of laboratory in the identification has gained greater attention as the incidence of fungal infection is increased. The objective of the study was to isolate the aflatoxin producing Aspergillus species from the rhizosphere of groundnut. Identification of the Aspergillus species was done on the basis of morphological species of Aspergillus namely A. flavus, A. parasiticus, A. niger, A. repens, A. fumigatus, A. kanagawaensis, A. ochraceus, A. sydowi, A. terreus and A. nidulans and were isolated and characterized. Rapid detection of aflatoxin production was carried out by using coconut agar medium. The colonies were detected under long-wave UV light by the blue-green fluorescence on the reverse side after 2 to 5 days of growth. Further the aflatoxin production capacity was screened using thin layer chromatographic technique. Depending upon the intensity of bands on TLC plates A. flavus and A. parasiticus were found to be highly aflatoxigenic and produce aflatoxin B1 on Richard liquid medium in the range of 966.66-266.66 μg/kg respectively. Quantitation of aflatoxin was done by using quantitative TLC, which was based on the comparison of the fluorescent intensity of the sample spot with standard spot. While all the other species are non-aflatoxigenic strains.

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