Vol. 8 & Issue 2 ; Section B : Biological Sciences

Date : Feb. to April2018


Complex treatment in patient with Wilson’s disease

M. Avramova, V. Zidarova, Kr. Markova, R. Cholakova, M. Drangov, St.Hristov  , Sv. Bachurska,

JCBPS; Section B; February 2018 – April 2018, Vol. 8, No. 2; 301-309,

[DOI: 10.24214/jcbps.B.8.1.30109.]

  • Abstract

    Wilson’s disease is an autosomal recessive disorder, caused by a mutation in the ATP 7B gene, it is a membrane-bound copper-transporting ATPase. The distorted copper excretion increases its serum concentration and leads to an accumulation within the body. Multi-organ manifestations are observed from the liver, the nervous system, kidneys, eyes and heart.
    Case report: We present a patient at the age of 46, with Wilson’s disease, she visited the clinical halls of FDM-Plovdiv, requiring complex treatment for the recovery of the chewing apparatus. A treatment plan was drawn up after diagnosis, including tooth extraction under local anesthesia, whereas she underwent a CBC and biochemistry with an INR test, due to the rest of hemorrhage in these patients. Material for a histological test was sampled during the extraction, aimed at establishing the presence of copper in the mucous membrane and bone. Recovery of speech, function and aesthetics was performed with detachable and fixed denture constructions that contain no copper. The disease is incredibly rare, approximately 1 in 40 000, with serious damage to the liver and the nervous system, which requires an in-depth analysis and discussion of the treatment plan, for its realization, as well as not allowing any complications during its implementation.

Mycotoxins and food preservation using essential oils extracted from plants grown in Rwanda


JCBPS; Section B; February 2018 – April 2018, Vol. 8, No. 2; 310-321

[DOI: 10.24214/jcbps.B.8.2.31021.]

  • Abstract

    We report the incidence of mycotoxins in some foodstuffs consumed in Rwanda and study on preservation of some of the analysed foodstuffs using naturally available products. Food samples were taken from various locations in Rwanda and analyzed for mycotoxins. Mycotoxin analysis was carried out by High Performance Liquid Chromatography tandem mass spectroscopy (HPLC-MS/MS, Agilent) and GCMS was used for essential oils chemical profiling. Preservation against mycotoxin producing fungi such as Aspergillus flavus, Aspergillus niger, Aspergillus parasiticus,Fusarium oxysporum and Penicilium digitatum was also attempted. Results showed that aflatoxins are the most frequent in the analysed foodstuff flours with a frequency of 100%, 89%, and 33% in soybean, wheat, and sorghum samples, respectively. The highest total concentrations of mycotoxins in wheat flours were 34.4 μg/kg, 215.8 μg/kg and 5.0μg/kg for aflatoxins, fumonisins and ochratoxin A, respectively. C. citratus essential oil was the most effective against R. nigricans (1.0mg/ml) and least effective against A. niger (5.2mg/ml). C. citratus essential oil showed lower minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values than O. gratissimum essential oil. A. flavus and A. parasiticus were used to evaluate the antiaflatoxin activity whose results showed that, at the concentration of 4 μl/ml of C. citratus essential oil showed a completed inhibition to aflatoxin production for A. flavus and A. parasiticus whereas the essential oil of O. gratissimum showed weak antiaflatoxigenic activity. Therefore, the essential oil of C. citratus can be considered as better potential food preservative against food spoilage fungi than the essential oil from O. gratissimum.

Effect of mutagen (benzyl-N-hydroxy carbamate) on mycological production of citric acid by Aspergillus niger NCIM-683

Subedar Yadav,

JCBPS; Section B; February 2018 – April 2018, Vol. 8, No.2; 322-328

[DOI: 10.24214/jcbps.B.8.2.0322-0328.]

  • Abstract

    The effect of mutagen (benzyl-N-hydroxy carbamate) on mycological production of citric acid by Aspergillus niger NCIM-683 has been assessed. It has been found that the mutagenic compound under trial has effect on mycological production of citric acid and enhances the yield of citric acid to an extent of 7.153 g/100ml which is 4.301% higher in comparison to control fermentor flasks i.e. 6.858 g/100ml in 8 days of optimum incubation period, 1.8 pH and 30 0C temperature with 20% (w/v) molasses solution.

Detection the two sex hormone digital values with human clinical medicine science in milk fish (Chanos chanos)

Nan- Hung Chen,

JCBPS; Section B; February 2018 – April 2018, Vol. 8, No. 2; 329-336

[DOI: 10.24214/jcbps.B.8.2.32936.]

  • Abstract

    The aims of this study were to detect the clinical chemical digital values of the two sex hormones in milk fish by treatment with the Chemiluminescecent Immunoassay (CLIA) of human medicine. The digital values of luteinizing hormone (LH) and follicle stimulating hormone (FSH) were detected.