Vol. 7 & Issue 4 ; Section A : Chemical Sciences

Date : Aug. to Oct.2017


Validated Derivative Spectrophotometric Methods for the Analysis of Lincomycin in Bulk and Dosage Forms

Shaza W. Shantier , Mohammed M. Elimam, Elrasheed A. Gadkariem

[DOI: 10.24214/jcbps.B.7.4.85156

  • Abstract

    Spectrophotometric methods (zero-order, first order and second order derivative) were evaluted for the analysis of lincomycin in bulk and pharmaceutical formulation. The first and second derivative methods were found more suitable for the analysis as zero order spectrum showed non-defined λmax. Regression analysis for first and second derivative obeyed Beer’s law over the concentration range 3-15μg/ml with good correlation coefficient (not less than 0.999). The two methods were proved to be simple and sensitive (LOD and LOQ: 0.676μg/ml and 2.28μg/ml, respectively). Selectivity was reflected by the obtained recovery percentage results (100.3 ± 1.3%; n=3).

Comparative in Vitro Evaluations of Different Brands of Atenolol Tablets in Libyan Market

Mohamed M. Siaan,Massud A. S. Anwair,, Anisa Elhamili3Fathi Sadek,

[DOI: 10.24214/jcbps.A.7.4.85767]

  • Abstract

    Continuation of our research studies of various pharmaceutical products in the Libyan market in order to monitor their quality, effectiveness, safety and safety of patients. So, in this study we focused on comparative in vitro evaluations of different brands of atenolol tablets and this study included some of the specifications that should be provided in medicine from appearance, thickness, diameter, weight variation, hardness, friability, disintegration time, dissolution, tensile strength, thickness and diameter, where these investigations have become widely accepted as a method of controlling of drug products and we have Prepared of standard calibration curve of atenolol by methanol for the assay and other standard calibration curve of atenolol by 0.1NHCL for the dissolution and the results data were compared them with scientific references and we found that all the visual inspection testing for all the atenolol tablets of the 3 brands were in a good condition, and each individual tablet was free from cracks and the tablets color, surface smoothness & polish were uniform on whole surface for the samples tested.

Iodometric Determination of Glucose using Chloramine-T

Vatsala U, Sumana Y. Kotian, Narayana U. Kudva N, Rajesh Acharya and K. M. Lokanatha Rai,

[DOI: 10.24214/jcbps.A.7.4.86870]

  • Abstract

    In this work, we have reported a simple, cost effective and reliable method for the determination of glucose iodometrically by making use of chloramine-T (CAT). This volumetric method determines glucose instantly, thereby greatly reduces the time of determination.

Thermodynamic Study of Mild Steel Corrosion Inhibition in Sulphuric Acid Medium by Tribulus terrestris Fruit Extract

Suchitra Chaudhary, Rakesh K. Tak

[DOI: 10.24214/jcbps.A.7.4.87181]

  • Abstract

    Tribulus terrestris fruit extract was studied for its inhibitory effect on corrosion of mild steel in different concentration of H2SO4 solution using mass loss method. The temperature effect on corrosion behavior with addition of plant extract was studied in temperature rang 303-333 K at 0.75N H2SO4. The inhibition efficiency increases with increase in extract concentration but decrease with increase in temperature. The adsorption mechanism of inhibition was supported by Langmuir adsorption isotherm. The obtained thermodynamic parameters revealed that adsorption of inhibitor on mild steel surface was endothermic, spontaneous and physiosorption mechanism.

Co(III) and Cu(II) benzimidazole Schiff base complexes: Synthesis, Characterization and DNA binding and cleavage

B. C. Vasantha Kumara, G. K. Lohithb, H. D. Revanasiddappa

[DOI: 10.24214/jcbps.A.7.4.88295]

  • Abstract

    Four new cobalt(III) and copper(II) complexes with 2-(5,6-Dihydrobenzo[ 4,5]imidazo[1,2-c]quinazolin-6-yl)-6-methoxy-phenol were synthesized and characterized by elemental and various spectral analysis. All the synthesized compounds were screened for their in vitro antibacterial activity against different strains of bacteria by disc diffusion method. The DNA interaction of the complexes was studied by spectroscopic, viscosity and electrophoretic measurement methods. The complexes were partially intercalated CT-DNA and cleaved pUC18 DNA efficiently via an oxidative

Synthesis, characterization DNA binding and biological activity of Copper(II) complexes with mixed ligands

Dharmalingam Ezhilarasan and M. N. Arumugham

[DOI: 10.24214/jcbps.A.7.4.89605.]

  • Abstract

    Copper complexes of the type [Cu(Phen)(L-Met)(TU)]NO3 (1) and [Cu(Bipy)(L-Met)(TU)]NO3 (2) (where Phen = 1,10-Phenanthroline, 2,2’=bipyridyl, L-Met=L-Methionine and TU=Thiourea), was synthesized and characterized by elemental analysis, UV-Visible, IR, and EPR studies. These compounds exist as distorted square pyramidal structures with five co-ordination sites occupied by the tridentate peptide dianion and the two heterocyclic nitrogens of the phenanthroline ligand. The bulk of the lateral chain in the peptide moiety determines the relative disposition of the phenanthroline ligand. The one-electron paramagnetic complexes display a d–d band near 600 nm in water. DNA Binding interactions of these complexes with calf thymus DNA have been examined by absorption, emission, cyclic voltammetry studies and viscosity. The results suggest that the ternary Copper (II) complexes bind to DNA through intercalation mode. The antimicrobial study indicates that these complexes have higher affinity against gram positive, gram negative bacteria and fungi.

Synthesis and characterization of copper-caffeine complex for anti-bacterial studies using MIC method

Patil Rajashree. I, Vidyasagar. C.C, Kishore G. Bhat

[DOI: 10.24214/jcbps.A.7.4.90618.]

  • Abstract

    The interaction of caffeine with divalent copper ions was studied in aqueous solution. The resulting complexes were found to be crystalline and used to determine the cation binding mode and association constants, which are characterized using X-ray diffraction studies (XRD), fourier transform infrared spectroscopy (FTIR) and absorption spectra (UV-Visible). The investigation on the study of interaction between caffeine and Cu2+ ions and their molecular behavior shows significant change in the molecular interactions through metal hydration shell. Especially ligand like caffeine can incredibly interact with metal and capable of altering the conformation of structure. The metal binding interaction of caffeine is quite useful to understand the molecular interaction with Cu2+ when they are targeted upon a bacterial growth. As a copper is an electron acceptor it trims down the capacity of growth of bacteria and fungus, which implies the reduction in bacterial growth. Aspergillus flavus fungus has the less capacity to substitute oxygen with alternative oxidisable compounds as terminal electron acceptor in respiration by showing highly sensitive up to 0.2 gm/L. However, biological implications point to the importance of knowing the characteristic of metal ion complexation in water solution and therefore more sensitive technique should be used.

  • Abstract

Method Development and Validation of Combination of Methocarbamol and Ibuprofen Using RP-HPLC

N.Ramalakshmi, Savita, C.N.Nalini and S.Arunkumar

[DOI: 10.24214/jcbps.A.7.4.91929.]

  • Abstract

    A new simple, rapid and sensitive isocratic RP-HPLC method has been developed for the determination of methocarbamol and ibuprofen. The method employs shimadzu HPLC system on ODS HG5 column. Best chromatographic separation was achieved by using orthophosphoric acid : methanol : Acetonitrile in the ratio of 15:45:40 as mobile phase with a flow rate of 1ml/min and isocratic elution with a total run time of 30 minutes. Detection of the compound was carried out at 230nm. The retention times of methocarbamol and ibuprofen were found to be 2.8 and 3.6 respectively. The linearity studies range from at the concentration range of 80 -120 μg/ml. This method was validated for accuracy, precision, linearity, ruggedness and robustness as per ICH guidelines. The present newly developed method was found to be accurate, precise and can be useful for routine Quality control analysis.

Chemical composition (C, N, P) of zooplankton from the Andaman Sea

I. Nageswara Rao* and V. Lakshmana Rao

[DOI: 10.24214/jcbps.A.7.4.93036.]

  • Abstract

    Zooplankton samples were collected from the surface waters of Andaman Sea during post-monsoon season from 22 stations. Higher zooplankton biomass, dry weight and populations were observed in the Andaman Sea area attributed with higher productivity in these waters than that of the waters of eastern Bay of Bengal due to higher availability of nutrients in these waters.. Powdered zooplankton samples were analyzed for elemental (C, N, P) composition. Carbon content as percent dry weight varies from 28.35 to39.88%, nitrogen content ranged from7.01 to 12.10% and the phousphorus content varied from 0.48 to 1.08%. Relatively higher values of carbon, nitrogen and phosphorus were observed from the zooplankton of Andaman Sea than that of eastern Bay of Bengal waters

Extraction and Bulk Liquid Membrane Transport of Alkali Metal Ions (Li+, Na+ and K+) By New Synthetic Podands

Loni Lokwani, Varsha Gautam and Sharda Sharma

[DOI: 10.24214/jcbps.A.7.4.93748.]

  • Abstract

    New podands were synthesized and used to study their extractability and carrier ability for alkali (Li+, Na+ and K+) metal ions using picrate, dinitrophenolate and orthonitrophenolate salts through dichloromethane, 1, 2-dichloroethane and chloroform membranes. Higher extraction and transport values were observed for K+ ions from picrate salts through dichloromethane membrane. Selectivity during extraction is found to be depending mainly on the size of cation, its solvation, dessolvation as well as structure, end group and chain length of podand. The membrane process is successfully applied in advance components of analytical instruments, biomedical and biological applications, textile and pharmaceutical industry.

  • Abstract

Corrosion Inhibition of Aluminium in Hydrochloric Acid by Lawsonia inermis Leaves Extract

R. T. Vashi and N. I. Prajapati

[DOI: 10.24214/jcbps.A.7.4.95064.]

  • Abstract

    The inhibitive action of Lawsonia inermis (Henna) leaves extract on corrosion of aluminium in hydrochloric acid solution was investigated using weight loss, potentiodynamic polarization and electrochemical impedance spectroscopic methods. The effect of inhibitor concentrations on different acid concentrations was investigated. The present study revealed that the percentage of inhibition efficiency is enhanced with increase of inhibitor concentration and decrease with increase in temperature. The inhibitive action of the extract is discussed in view of adsorption of Lawsonia inermis molecule on the metal surface. It was found that the adsorption follows Langmuir adsorption isotherm. Tafel plot of polarization study indicate that the Lawsonia inermis leaves extract acts as a mixed type inhibitor. Inhibition efficiency of Lawsonia inermis leaves extract was found up to 85.34 % at 1.2 g/L concentration in 0.75 M HCl solution. The results obtained showed that the leaves extract of Lawsonia inermis could serve as an effective green inhibitor for corrosion of aluminium in hydrochloric acid.

Phytoremediation Potentials of Sunflower (Helianthus annus) on Soil Contaminated with Organochlorine Pesticide Residue

S Garba and L Dauda

[DOI: 10.24214/jcbps.A.7.4.96573.]

  • Abstract

    The physicochemical properties of IAR soil and the phytoremediation potential of Helianthus annus (Sunflower) plant on organochlorine pesticide residue were determined using standard methods. Results of the physicochemical analyses showed that IAR soil had a pH range of 4.73 – 4.93, organic matter content range of 0.71 – 2.39 and particle size range of: 10% - 12% of clay, 42% - 46% of silt and 44% - 46% of sand. Results of the absorption of organochlorine pesticide residue showed that Sunflower absorbed a mean concentration of 3.4556 + 3.0174 mg/kg of alpha-BHC, 0.4873 + 0.3638 mg/kg of beta-BHC, 3.4884 + 6.0400 mg/kg of Heptachlor, 2.2781 + 1.86238 mg/kg of Aldrin, 1.4494 + 1.3549 mg/kg of gamma-BHC, 6.7012 + 5.7216 mg/kg of delta-BHC, 37.3056 + 19.9992 mg/kg of Heptachlor-epoxide, 88.8459 + 72.9678 mg/kg of Endosulfan I, 5236.2737 + 0 mg/kg of Dieldrin, 0.9224 + 0 mg/kg of Endrin , 21.7438 + 21.4416 mg/kg of p,p’ DDD, 12.5867 + 0 mg/kg of Endosulfan II, 13.0872 + 12.9082 mg/kg of p,p’-DDT, 1858.8949 + 1816.4019 mg/kg of Endrin Aldehyde, 852.0080 + 1147.7685 mg/kg of Endosulfan sulphate and 16.5957 + 19.2893 mg/kg of Methoxychlor. The results of this work showed that sunflower could be used for the absorption of organochlorine pesticides residue especially on loamy soils.

Preparation, characterization and application of green coconut shell (Cocos nucifera) extract based azo dye

Ayesha Akhter, Most. Halima Khatun, H. Jahan Kadri, Bijoy Maitra, M. Mehedi Hasan and M. Ahasanur Rabbi

[DOI: 10.24214/jcbps.A.7.4.97478.]

  • Abstract

    Environmental and toxicological concerns have resulted in increased interests in new diazotization reaction for the preparation of new azo compound. In this research work new azo dye has been prepared and aqueous extract of green coconut (Cocos nucifera) shell was used as a source of polyphnolic compounds. Green coconut grows abundantly in all over of Bangladesh and after taking the soft water the most of the coconut shell are discarded. Aqueous extract of green coconut shell was used to synthesize a new azo dye through diazotization reaction and the synthesized dye was characterized by FTIR and UV-VIS spectroscopy. Solubility of the dye was examined and found to soluble in organic solvents. The azo dye can be used as wood stain and along with shellac varnish gives an orange-red color to the wood coating.